TREATMENT EFFICACY

BioSafeFood project 

“Development of high quality fruits and vegetables production technology, safe for consumers, with the use of new biopreparations to protect crops against diseases.”

 

The aim of the project:

The main objective of the project is to develop and introduce into production of fruits and vegetables new, safe biopreparations protecting against the most important diseases, and to develop new technologies for the production of fruits and vegetables with the use of these means.

The main elements of the technology will be 3 groups of bioproducts intended for protection:

1. Berry plants, vegetables and fruit trees against grey mould.

2. Apples and pears, among others, against fire blight and apple scab.

3. Fruit and vegetables against diseases in the post-harvest period.

 

Our activities are in line with the current priorities of the European Union to produce fruit without residues of chemical plant protection products and to reduce the risk of environmental pollution.

 

The project's consortium members are:

Financing:

The National Centre for Research and Development. Smart Growth Operational Program 2014-2020.

 

Contact:

PhD Katarzyna Góralska (katarzyna.goralaska@intermag.pl), Małgorzata Hałat-Łaś (malgorzata.halat-las@intermag.pl), Marcin Oleszczak (marcin.oleszczak@intermag.pl).

 

1. Berry plants, vegetables and fruit trees against grey mould.

 

Apples flowers.

Flowers on the trees in the greenhouse were sprayed with a suspension of bacteria (108 cfu/ml) and then after 24 hours they were infected with a spore suspension of Botrytis cinerea (105 cfu/ml).

 

Results of our study.

Treatments Infected flowers 
                   Untreated (water)                 100%
Our isolate  5%

 

Grape fruit.

The grape fruit were inoculated with the bacterial suspension (107 cfu/ml) under laboratory conditions and after 24 hours grapes were infected with a spore suspension of Botrytis cinerea  (106 cfu/ml).

 

Results of our study.

Treatments

Efficacy after 7 days

                      Untreated (water)              0%
Our isolate (10cfu/ml) 65%

 

Efficacy our isolate against anthracnose on blueberry fruit.

Blueberry fruits were inoculated with bacterial suspension (107 cfu/ml) under laboratory conditions and after 24 hours fruits were infected with Colletotrichum sp. suspension (106 cfu/ml).

 

Results of our study.

Treatments Efficacy
after 4 days after 6 days
Untreated (water) 0% 0%
Our isolate (107 cfu/ml) 75% 50%

 

2. Apples and pears, among others, against fire blight and apple scab.

 

Erwinia amylovora control  experiment.

 

Pear fruit.

Under control conditions the pear fruit slices were treated by water suspension of tested isolate. As an active control were used commercial chemical product (copper oxychloride) and  biological product (Pantoea agglomerans C9-1).

After 6 hours the slices were inoculated with E. amylovora (strain Ea 659) by spraying water suspension (107 cfu/ml).

 

Results of our study.

Treatments Efficacy
after 4 days after 7 days
Untreated (water) 0% 0%
Chemical product (1.5%) 61.2% 37.5%
Our isolate (107 cfu/ml) 74.9% 49.3%
Biological product* (107 cfu/ml) 71.1% 45.0%

*Pantoea agglomerans – active ingredient of biological product.

 

Apple flowers.

The apple trees in the greenhouse were sprayed with water suspension of the bacterial isolate (108 cfu/ml or 107 cfu/ml) during full blooming. As an active control were used commercial chemical product (copper oxychloride) and  biological product (Pantoea agglomerans C9-1).

 

Results of our study.

Treatments Efficacy
after 7 days after 10 days
Untreated (water) 0% 0%
Chemical product (1.5%) 82.4% 57.6%
Our isolate (107 cfu/ml) 92.2% 88.8%
Biological product* (107 cfu/ml) 90.8% 82.9%

*Pantoea agglomerans – active ingredient of biological product.

 

Apple shoots.

Actively growing shoots were cut off and then were sprayed with water suspension of bacteria (107 cfu/ml). As chemical standard was used commercial product (copper oxychloride) and as biological standard  was used product with Pantoea agglomerans (C9-1). Inoculation was performed 5 hours after treatment by spraying with water suspension of Erwinia amylovora (strain Ea 659)  at concentration 107 cfu/ml.

When the first symptoms on shoots were visible the measurement of the length of necrotized part of shoots was made.

 

Results of our study.

Treatments Efficacy
after 6 days after 10 days after 14 days
Untreated  0% 0% 0%
Chemical product (1.5%) 61.7% 51.7% 43.8%
Our isolate (107 cfu/ml) 65.5% 62.8% 62.5%
Biological product* (107 cfu/ml) 60.1% 55.5% 58.8%

*Pantoea agglomerans – active ingredient of biological product.

 

3. Fruit and vegetables against diseases in the post-harvest period.

 

Protective properties of bacteria against grey mould and white mould on cabbage.

Cabbage leaves were soaked in bacterial suspension for 5 minutes. After the dried, the leaves were inoculated with agar plug from 5-day culture of pathogen.

 

Results of our study.

Treatment Efficacy after 7 days
Botrytis cinerea
Our isolate 1 41,2%
Our isolate 2 52,0%
Our isolate 3 50,0%
Our isolate 4 70,6%
Our isolate 5 64,6%
Our isolate 6 69,0%
Our isolate 7 67,0%
Our isolate 8 65,2%
Our isolate 9 55,6%
Our isolate 10 75,2%
Our isolate 11 66,3%
Our isolate 12 95,5%
Our isolate 13 58,7%
Our isolate 14 80,7%
Sclerotinia sclerotiorum
Our isolate 15 70,0%
Our isolate 16 83,2%
Our isolate 17 55,7%
Our isolate 18 66,9%
Our isolate 19 99,2%
Our isolate 20 53,7%

 

Protective properties of bacteria on apples.

In apples fruit were made holes and then a bacterial suspension was added to them or water in the case of a control combination.

Apples were infected with a spore suspension of Neofabraea alba, Botrytis cinerea.

 

Results of our study.

Our isolates Efficacy % – Neofabraea alba
Strain A Strain B
1 86,0 100
2 94,0 100
3 76,0 88,0
4 100 97,2

 

Our isolates Efficacy % – Botrytis cinerea
Strain A Strain B
1 99,5 100
2 100 100
3 97,4 100
4 93,8 92,5